Part:BBa_K3657037
RNA Linker binding Pumby AUAGAU and Pumby AUGGUU
This part is an RNA containing binding sites for Pumby protein binding AUAGAU (BBa_K3657004) and Pumby protein binding AUGGUU (BBa_K3657005).
Usage and Biology
Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide. Therefore the protein can be designed to bind a specific RNA sequence.
It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: BBa_K3657034, BBa_K3657035.
Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: iGEM Heidelberg 2020
Figure 1: Secondary structure of the part.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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